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    大鼠腫瘤壞死因子α(TNFa)酶聯免疫吸附檢測試劑盒
    ELK1396
    規格: 價格:
    48T ¥1680.00
    96T ¥2400.00

    Overview 文獻

    Product name: Rat TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
    Reactivity: Rat
    Alternative Names: TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
    Assay Type: Sandwich
    Sensitivity: 6.1 pg/mL
    Standard: 1000 pg/mL
    Detection Range: 15.63-1000 pg/mL
    Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay Length: 3.5h
    Research Area: Cytokine;Tumor immunity;Infection immunity;
    Uniprot ID: P16599
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    1000.00 2.345 2.256
    500.00 1.769 1.680
    250.00 1.225 1.136
    125.00 0.917 0.828
    62.50 0.521 0.432
    31.25 0.366 0.277
    15.63 0.207 0.118
    0.00 0.089 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant TNFa and the recovery rates were calculated by comparing the measured value to the expected amount of TNFa in samples.
    Matrix Recovery range Average
    serum(n=5) 80-90% 85%
    EDTA plasma(n=5) 84-106% 100%
    Heparin plasma(n=5) 87-93% 90%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 89-97% 85-94% 87-96% 85-97%
    EDTA plasma(n=5) 85-94% 87-96% 92-101% 82-98%
    Heparin plasma(n=5) 85-99% 98-106% 86-99% 78-95%
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