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    一步法人骨保護素(OPG)酶聯免疫吸附檢測試劑盒
    ELK034ES
    規格: 價格:
    48T ¥1960.00
    96T ¥2800.00

    Overview

    Product name: EasyStep Human OPG(Osteoprotegerin) ELISA Kit
    Reactivity: Human
    Alternative Names: TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
    Assay Type: Sandwich
    Sensitivity: 23.6 pg/mL
    Standard: 2000 pg/mL
    Detection Range: 31.25-2000 pg/mL
    Sample Type: Serum
    Assay Length: 1.5h
    Research Area: Cytokine;Metabolic pathway;Bone metabolism;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG, and the Human OPG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OPG. After TMB substrate solution is added, only those wells that contain Human OPG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    2000.00 2.154 2.072
    1000.00 1.534 1.452
    500.00 1.135 1.053
    250.00 0.847 0.765
    125.00 0.535 0.453
    62.50 0.386 0.304
    31.25 0.175 0.093
    0.00 0.082 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant OPG and the recovery rates were calculated by comparing the measured value to the expected amount of OPG in samples.
    Matrix Recovery range Average
    serum(n=5) 80-96% 88%
    EDTA plasma(n=5) 87-99% 93%
    Heparin plasma(n=5) 83-99% 95%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 87-96% 85-97% 85-94% 87-96%
    EDTA plasma(n=5) 93-102% 93-106% 87-101% 91-104%
    Heparin plasma(n=5) 89-97% 93-102% 81-96% 93-106%

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