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    一步法人血管內皮生長因子A(VEGFA)酶聯免疫吸附檢測試劑盒
    ELK033ES
    規格: 價格:
    48T ¥1960.00
    96T ¥2800.00

    Overview

    Product name: EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
    Reactivity: Human
    Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
    Assay Type: Sandwich
    Sensitivity: 31 pg/mL
    Standard: 5000 pg/mL
    Detection Range: 78.2-5000 pg/mL
    Sample Type: Serum, plasma
    Assay Length: 1.5h
    Research Area: Cytokine;Apoptosis;Tumor immunity;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

    標準曲線

    Concentration (pg/mL) OD Corrected OD
    5000.00 2.266 2.183
    2500.00 1.674 1.591
    1250.00 1.243 1.160
    625.00 0.845 0.762
    312.50 0.585 0.502
    156.25 0.293 0.210
    78.13 0.179 0.096
    0.00 0.083 0.000

    精密度

    Intra-assay Precision (Precision within an assay):CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    回收率

    Matrices listed below were spiked with certain level of recombinant VEGFA and the recovery rates were calculated by comparing the measured value to the expected amount of VEGFA in samples.
    Matrix Recovery range Average
    serum(n=5) 80-95% 87%
    EDTA plasma(n=5) 80-95% 87%
    Heparin plasma(n=5) 86-99% 92%

    線性

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 98-105% 92-101% 85-105% 87-98%
    EDTA plasma(n=5) 88-104% 84-98% 95-105% 88-102%
    Heparin plasma(n=5) 88-95% 98-107% 90-99% 88-97%

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